- JOB
- Netherlands
Job Information
- Organisation/Company
- Utrecht University
- Research Field
- Medical sciences
- Researcher Profile
- First Stage Researcher (R1)
- Country
- Netherlands
- Application Deadline
- Type of Contract
- Temporary
- Job Status
- Not Applicable
- Hours Per Week
- 40.0
- Is the job funded through the EU Research Framework Programme?
- Not funded by a EU programme
- Is the Job related to staff position within a Research Infrastructure?
- No
Offer Description
We are seeking a highly motivated PhD candidate to join our multidisciplinary team as part of the recently funded ERC Starting Grant project PicAAA. This project aims to elucidate the role of virally encoded AAA+ ATPases in the replication cycles of positive-strand RNA viruses, with a particular emphasis on Noroviruses.
Your job
Noroviruses are the leading cause of non-bacterial gastroenteritis outbreaks worldwide. Despite their substantial global health impact, our molecular understanding of Norovirus replication remains incomplete. Critically, no approved direct-acting antivirals are currently available to protect high-risk populations from severe or life-threatening disease.
As a key step toward addressing this gap, our team recently published a preprint presenting the first high-resolution structure of the Norovirus NS3 protein, revealing a putative transmembrane RNA translocase:
🧬 Haas et al. (2025) – Integrative Structure of Norovirus NS3 Suggests a Role in RNA Transport
https://www.biorxiv.org/content/10.1101/2025.06.17.659504v1
Building on this foundation, the PhD candidate will use cutting-edge structural biology approaches—primarily single-particle cryo-electron microscopy (cryo-EM)—to investigate the mechanistic role of NS3 in viral replication and assembly. The project will also explore how NS3 activity may be disrupted by small-molecule inhibitors. Additionally, the candidate will have opportunities to apply in situ cryo-electron tomography to visualize NS3 function and replication dynamics within infected cells.
Key Responsibilities
- Express and purify recombinant Norovirus NS3 complexes for biochemical and structural studies
- Design and carry out single-particle cryo-EM experiments to visualize NS3 complexes at high resolution
- Support antiviral compound screening and contribute to the structural characterization of NS3–inhibitor complexes
- Process and analyze cryo-EM data using established computational pipelines and structural modeling tools
- Apply, or develop expertise in, in situ cryo-electron tomography to investigate NS3 function and viral replication within infected cells
- Collaborate closely with molecular virologists, structural biologists, and computational scientists within an interdisciplinary team
- Supervise and mentor Bachelor’s and Master’s students involved in related research projects
- Communicate findings through peer-reviewed publications and presentations at international scientific conferences
Where to apply
Requirements
- A Master’s degree in Biochemistry, Virology, Molecular Biology, Structural Biology, or a related discipline.
- Hands-on experience with protein expression and purification, ideally involving recombinant membrane or viral proteins.
- A strong interest in virus–host interactions, viral replication mechanisms, and antiviral drug development.
- Experience with, or a clear motivation to learn, cryo-EM data collection, image processing, and 3D structural analysis.
- Prior experience with cryo-EM single-particle analysis (SPA) and/or cryo-electron tomography (cryo-ET) is a strong advantage, but not a requirement.
- Background knowledge in virology or protein biochemistry is beneficial.
- Ability to drive projects independently as well as part of a collaborative, interdisciplinary research team.
- Strong problem-solving skills, a well-organised approach to research, and excellent written and verbal communication abilities.
- Enthusiasm for mentoring undergraduate or Master’s students and contributing to a supportive research environment.
- Motivation to stay up to date with relevant literature in virology, structural biology, and antiviral development.
Additional Information
- A fully funded, four-year PhD position within an internationally recognized virology and structural biology laboratory.
- a working week of 36 - 40 hours and a gross monthly salary between € 2.901 and €3.707 in the case of full-time employment (salary scale P under the Collective Labour Agreement for Dutch Universities (CAO NU));
- 8% holiday pay and 8.3% year-end bonus;
- a pension scheme, partially paid parental leave and flexible terms of employment based on the CAO NU.
In addition to the terms of employment laid down in the CAO NU, Utrecht University has a number of schemes and facilities of its own for employees. This includes schemes facilitating professional development, leave schemes and schemes for sports and cultural activities, as well as discounts on software and other IT products. We also offer access to additional employee benefits through our Terms of Employment Options Model. In this way, we encourage our employees to continue to invest in their growth. For more information, please visit Working at Utrecht University.
As Utrecht University, we want to be a home for everyone. We value staff with diverse backgrounds, perspectives and identities, including cultural, religious or ethnic background, gender, sexual orientation, disability or age. We strive to create a safe and inclusive environment in which everyone can flourish and contribute.
To apply, please send your curriculum vitae, including a letter of motivation, via the ‘apply now’ button.
For more information, please contact Dr. Daniel Hurdiss at d.l.hurdiss@uu.nl.
Candidates for this vacancy will be recruited by Utrecht University.
- Website for additional job details
Work Location(s)
- Number of offers available
- 1
- Company/Institute
- Universiteit Utrecht
- Country
- Netherlands
- City
- Utrecht
- Postal Code
- 3584CL
- Street
- Yalelaan 1
- Geofield
Contact
- City
- Utrecht
- Website
- Street
- Domplein 29
- Postal Code
- 3512 JE